The membrane of gram-negative bacteria is not thick, but it is still nearly impossible to penetrate.Īll in all, gram-positive bacteria have a cell membrane about 20-fold thicker as compared to the protective membrane of gram-negative bacteria. What it implies is that gram positive bacteria have a thick outer covering, which can absorb foreign material with ease. The wall of gram positive bacteria is like a heavy, thick wooden fence, whereas the wall of gram-negative bacteria is more like a thin bulletproof Kevlar vest. Gram-negative bacteria are usually much harder to kill, and here comes more. Their structure tells a lot about how hard it is to kill them. Making a comparison of gram-positive and gram-negative bacteria helps find information about how these bacteria behave. It does not contain techoic acid but lipid content is between 20 and 30%. It comes in contact with plasma membrane at a few loci. The wall has 70-80% murein with low lipid content. The thickness of cell wall is 20-30 nm, and it is smooth. gram negative bacterial, there are some obvious differences. Other Differences Between the Two Types of Bacteria This mainly happens because their difference in the structure of cell walls: there is no outer cell membrane in Gram-negative bacteria however, the cell wall of Gram-positive bacteria is different and is high in peptidoglycan. This changes Gram-negative bacteria to pink or red, while Gram-positive bacteria maintain their violet dye. After washing, a counterstain is added, which is usually fuchsine or safranin. One way to classify them is to perform Gram staining test to check if they belong to gram positive category or are gram negative bacteria.ĭuring a Gram stain test, bacteria are dyed crystal violet and then are washed with a decolorizing solution. Gram Negative Reactionĭifferent types of bacteria have been identified already, but they are classified differently. The same method of "Gram staining" is still in use to distinguish between gram positive and gram negativebacteria. It was in 1884 when Hans Christian Gram, a Danish bacteriologist, devised a test and introduced a dye to the bacteria to check if bacteria had a peptidoglycan wall or simply a mesh-like layer of amino acids and sugars.
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